Aquaplates™ m-TEC Agar Media Plates (for conventional Membrane Thermotolerant E coli)

Aquaplates™ m-TEC Agar Media Plates (for conventional Membrane Thermotolerant E coli)
This is an earlier two-step process in which the filer membrane is first placed on prepared agar plates, incubated as in the modified process but then after 24 hours, is transferred over pads saturated with urea and phenol red solutions. Presence of E coli is indicated by yellow to brown color in about 15-20 minutes. Note that most people now prefer a simpler one-step Modified m-TEC agar plates featured here.

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Steps for Fecal Coliform Testing


 Step 1. Filter the sample with a sterile cellulosic 0.45 micron filter.


 Step 2. With flamed (sterile) tweezers, gently place the filter on the modified m-TEC media plates by placing it on one end rolling it to the other edge.

 Step 3. Incubate this plate at 44.5 degrees Celcius for 24 (+/- 2 hours).

 Step 4. That’s it. You are done!. Simply count the colonies which are blue in color.

An added advantage of these prepared media plates is that it incorporates rosolic acid which discourages growth of competing organisms (e.g. E. coli and non-fecal coliform)


 Eliminates the hassle of dissolving and preparing agar gel and is a big time saver
 Provide reproducible, consistent results every time, saving time and money
 Come prepared and sealed in a plastic tube with expiration date clearly stamped on each plate. No need to buy plates with pads, no lights, substrates, or messy vials
 Produces easy to see blue colonies
 Very economical! Moreover, they also save money by providing consistency and reliability to your test
making the results more reliable
 Complete satisfaction guarantee
 Two months shelf-life


 Norweco Laboratory article on Membrane Fecal Coliform Test
 Article from Virginia Polytechnic Institute on Membrane Fecal Coliform Test

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